PCR Lab
PCR Lab
- The molecular laboratory aims to perform different techniques for investigating eye diseases such as glaucoma and diabetic retinopathy.
- Step one polymerase chain reaction system Support any real Time PCR applications such as:
- SNP genotyping
- Gene expression profiling.
- Micro RNA expression.
- Translocation analysis.
- Gene detection.
- The laboratory performs different techniques and experiments for investigation of glaucoma and diabetic retinopathy:
- Circulating angiogenic microRNA-27b and thrombospondin-1(TSP-1)in patients with diabetic retinopathy.
- RIoGlaucoma screening programme for primary open angle glaucoma(POAG).
- RIO programme for prediction of diabetic retinopathy in Ezgyptian patients with type I diabetes mellitus.
- Prevention of blindness from corneal opacity by using stem cells.
- Role of microRNA200b in the development of diabetic retinopathy by modulation of it’s target VEGF gene.
- Vascular endothelial growth factor (VEGF)+405G/C polymorphism as a predictor of diabetic retinopathy.
PCR Lab is a part of The Human Ophthalmic Genetic Unit, which includes:
1) The Molecular Biology Lab:
The lab do some molecular tests as:
- DNA Extraction from blood samples.
- DNA Extraction + PCR one site.
- PCR one site with restriction enzyme .
2) The Human Ophthalmic Genetic Clinic:
- Examining the genetic syndromes of the eye as Down syndrome and other chromosomal abnormalities and metabolic disorders.
- We also examine isolated cases of Cataract, Glaucoma, Corneal dystrophies, Keratoconus, Diabetic retinopathy and Retinal dystrophies.
